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USF Mass Spectrometry / Peptide Facility (USFMASP)


Mohanraja Kumar, Ph.D.
kumarm@usf.edu
Phone: (813) 974-6031
Fax: (813) 974-3203


Agilent 6540 LC/QTOF

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Mass Range/Resolution: - Range 40k m/z; Accuracy 20 – 10,000)
  • Spectrum Dynamic range: - 5 order of magnitude
  • Scan Speed: - 50 Spectra/sec
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolomic identification
  • Software: - Mass Hunter Workstation, Mass Profiler Professional (MPP)


Agilent 6460 LC/QQQ

  • Ionization: - Agilent Jet-stream Electrospray Ionization (ESI ± ve) and thermal gradient
  • Sensitivity: - High sensitivity (Femtomole to Attomole range)
  • Inlet system: - Agilent 1260 HPLC
  • Analytes: - Organic, Peptides, Proteins, Natural products, metabolomics and metabolite identification
  • Software: - Mass Hunter workstation
  • Studies: - Target ion capability (Quantitative analysis)


Agilent LC/MSD

  • Ionization: - Electrospray (ESI ± ve); Atmospheric pressure chemical ionization (APCI ± ve)
  • Mass range: - 10 – 1500 m/z
  • Inlet system: - Agilent 1100 HPLC with Diode Array Detector (DAD)
  • Analytes: - Organic, peptides, Natural products and non-polar compounds


Dionex Summit HPLC Systems

  • Analytical
    Automated injection and fraction collection, quantification of peaks and dual wavelength monitoring.
  • Preparative
    Manual injection and automated collection, dual wavelength monitoring and peaks quantification.


Symphony Peptide Synthesizer

  • Peptide synthesizer with 12 single channels
  • Synthesis scale 20 – 100 μmole / Channel
  • Peptide length 3 to 50 residues
  • Automated cleavage
  • Customized protocols


AVIV Model 215 Circular Dichroism Spectrometer

  • Records wavelength, time, temperature, pH and Concentration
  • Wavelength range 160 nm to 850 nm
  • Light Source: - Air cooled, 150 watt suprasil Xenon lamp controlled by a high stability, constant current, DC power supply
  • Cuvette size: - 1 cm and 1 mm path length